General Information
| Application | ELISA |
|---|---|
| Assay principle | This kit uses Competitive-ELISA as the method. It can detect β-agonist in samples, such as tissues, urine, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the detection, β-agonist in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti- β-agonist antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of β-agonist. The concentration of β-agonist in the samples can be calculated by comparing the OD of the samples to the standard curve. |
| Sensitivity | 0.2 ppb (ng/mL) |
| Storage instruction | Store at 2~8℃ for 1 year. Avoid freeze. |
| Reaction mode |
25℃, 15 min~15 min ~15 min |
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| Detection limit |
Urine --- 1 ppb; Muscle --- 1 ppb |
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| Cross-reactivity |
|
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|
Clenbuterol |
100% |
|
Clorprenaline |
141% |
|
Tulobuterol |
80% |
|
Brombuterol, Mapenterol |
107% |
|
Cimbuterol |
86.9% |
|
Clenpenterol |
78.3% |
|
Terbutaline |
60.6% |
|
Salbutamol |
60.6% |
|
Mabuterol |
56.3% |
|
Bambuterol |
18% |
|
Cimaterol |
14% |
|
Clenproperol |
5% |
|
Ractopamine, Zilpaterol, Phenylethanolamine A |
＜1% |
| Sample recovery rate |
90%±30% |
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| Kit components |
|
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|
Item |
Specifications |
|
ELISA Microtiter Plate |
96 wells |
|
Standard Liquid |
1 mL each (0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb) |
|
HRP Conjugate |
12 mL |
|
Antibody Working Solution |
7 mL |
|
Substrate Reagent A |
6 mL |
|
Substrate Reagent B |
6 mL |
|
Stop Solution |
6 mL |
|
Sample Stabilizer |
5 mL |
|
Sample Solution |
30 mL |
|
20×Concentrated Wash Buffer |
25 mL |
|
Plate Sealer |
3 pieces |
|
Sealed Bag |
1 piece |
|
Manual |
1 copy |
| Note |
All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. |
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| Other materials required but not supplied | Instrument: Microplate Reader, Homogenizer, Oscillators, Centrifuge, Balance (sensibility 0.01 g). Micropipettor: Single-channel (20-200 μL, 100-1000 μL), Multi-channel (300 μL). Reagents: Trichloroacetic acid, NaOH |
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