COVID-19 , COVID-19 (SARS-CoV-2) Detection kits, COVID-19 Pseudovirus, Pseudovirus, SARS-CoV-2

Pseudovirus-MERS-abEN

Catalog Number : PV021
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Introduction

The partial ORF1 a, ORF1, UP-E gene sequence and E gene, N gene full sequence were cloned into retroviral vector by chemical synthesis to obtain FNV-SARS-CoV-2-abEN pseudovirus. The pseudovirus are prepared with using 293T. For the concentration and purification of the ultracentrifuge. For the FNV-MERS-abEN pseudovirus we used ultracentrifugation. Viral envelope of the FNV-MERS-abEN pseudovirus include ORF1 a, ORF1, UP-E gene sequence and E gene, N gene full sequence, using in experiments which are related to RNA extraction and become the positive control of qPCR.

General Information

Formula	>₁ x ₁₀₇ copy/ml in ₁ ml
Storage buffer	glucose、KH2PO4、Na2HPO4、NaCl、KCl、FNV-MERS-abEN pseudovirus
Storage instruction	-20°C for 6 months

1.MERS-abEN

ACTACTTGTTTGAGACTATTCCCACACAGTTGTTCCCACT

CTTATTTGTGACTATGGCCTTCGTTATGTTGTTGGTTAAA

CACAAACACACCTTTTTGACACTTTTCTTGTTGCCTGTGG

CTATTTGTTTGACTTATGCAAACATAGTCTACGAGCCCAC

TACTCCCATTTCGTCAGCGCTGATTGCAGTTGCAAATTGG

CTTGCCCCCACTAATGCTTATATGCGCACTACACATACTG

ATATTGGTGTCTACATTAGTATGTCACTTGTATTAGTCAT

TGTAGTGAAGAGATTGTACAACCCATCACTTTCTAACTTT

GCGTTAGCATTGTGCAGTGGTGTTGGGCTTTAAACTCGAT

GCAACAGTTCCTGGATATCCTAAGCTTTTCATTACTCGTG

AAGAGGCTGTAAGGCAAGTTCGAAGCTGGATAGGCTTCGA

TGTTGAGGGTGCTCATGCTTCCCGTAATGCATGTGGCACC

AATGTGCCTCTACAATTAGGATTTTCAACTGGTGTGAACT

TTGTTGTTCAGCCAGTTGGTGTTGTAGACACTGAGTGGGG

TAACATGTTAACGGGCATTGCTGCACGTCCTCCACCAGGT

GAACAGTTTAAGCACCTCGTGCCTCTTATGCATAAGGGGG

CTGCGTGGCCTATTGTTAGACGACGTATAGTGCAAATGTT

GTCAGACACTTTAGACAAATTGTCTGATTACTGTACGTTT

GTTTGTTGGGCTCATGGCTTTGAATTAACGTCTGCATCAT

ACTTTTGCAAGATAGGTAAGGAACAGAAGTGTTGCATGTG

CACCACTGTTTTCGTGCCTGCAACGCGCGATTCAGTTCCT

CTTCACATAATCGCCCCGAGCTCGCTTATCGTTTAAGCAG

CTCTGCGCTACTATGGGTCCCGTGTAGAGGCTAATCCATT

AGTCTCTCTTTGGACATATGGAAAACGAACTGTTACCCTT

TGTCCAAGAACGAATAGGGTTGTTCATAGTAAACTTTTTC

ATTTTTACCGTAGTATGTGCTATAACACTCTTGGTGTGTA

TGGCTTTCCTTACGGCTACTAGATTATGTGTGCAATGTAT

GACAGGCTTCAATACCCTGTTAGTTCAGCCCGCATTATAC

TTGTATAATACTGGACGTTCAGTCTATGTAAAATTCCAGG

ATAGTAAACCCCCTCTACCACCTGACGAGTGGGTTTAAGG

CATCCCCTGCTGCACCTCGTGCTGTTTCCTTTGCCGATAA

CAATGATATAACAAATACAAACCTATCTCGAGGTAGAGGA

CGTAATCCAAAACCACGAGCTGCACCAAATAACACTGTCT

CTTGGTACACTGGGCTTACCCAACACGGGAAAGTCCCTCT

TACCTTTCCACCTGGGCAGGGTGTACCTCTTAATGCCAAT

TCTACCCCTGCGCAAAATGCTGGGTATTGGCGGAGACAGG

ACAGAAAAATTAATACCGGGAATGGAATTAAGCAACTGGC

TCCCAGGTGGTACTTCTACTACACTGGAACTGGACCCGAA

GCAGCACTCCCATTCCGGGCTGTTAAGGATGGCATCGTTT

GGGTCCATGAAGATGGCGCCACTGATGCTCCTTCAACTTT

TGGGACGCGGAACCCTAACAATGATTCAGCTATTGTTACA

CAATTCGCGCCCGGTACTAAGCTTCCTAAAAACTTCCACA

TTGAGGGGACTGGAGGCAATAGTCAATCATCTTCAAGAGC

CTCTAGCTTAAGCAGAAACTCTTCCAGATCTAGTTCACAA

GGTTCAAGATCAGGAAACTCTACCCGCGGCACTTCTCCAG

GTCCATCTGGAATCGGAGCAGTAGGAGGTGATCTACTTTA

CCTTGATCTTCTGAACAGACTACAAGCCCTTGAGTCTGGC

AAAGTAAAGCAATCGCAGCCAAAAGTAATCACTAAGAAAG

ATGCTGCTGCTGCTAAAAATAAGATGCGCCACAAGCGCAC

TTCCACCAAAAGTTTCAACATGGTGCAAGCTTTTGGTCTT

CGCGGACCAGGAGACCTCCAGGGAAACTTTGGTGATCTTC

AATTGAATAAACTCGGCACTGAGGACCCACGTTGGCCCCA

AATTGCTGAGCTTGCTCCTACAGCCAGTGCTTTTATGGGT

ATGTCGCAATTTAAACTTACCCATCAGAACAATGATGATC

ATGGCAACCCTGTGTACTTCCTTCGGTACAGTGGAGCCAT

TAAACTTGACCCAAAGAATCCCAACTACAATAAGTGGTTG

GAGCTTCTTGAGCAAAATATTGATGCCTACAAAACCTTCC

CTAAGAAGGAAAAGAAACAAAAGGCACCAAAAGAAGAATC

AACAGACCAAATGTCTGAACCTCCAAAGGAGCAGCGTGTG

CAAGGTAGCATCACTCAGCGCACTCGCACCCGTCCAAGTG

TTCAGCCTGGTCCAATGATTGATGTTAACACTGATTAG

ORF1a:CCACTACTCCCATTTCGTCAG/CAGTATGTGTAGTGCGCATATAAGCA;

ORF1b:TCGATGTTGAGGGTGCTCAT/ACACCAGTTGAAAATCCTAATTG;

Up-E CAACGCGCGATTCAGTT/GCCTCTACACGGGACCCATA

Pseudovirus-MERS-abEN

Introduction

General Information

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Pseudovirus-MERS-abEN

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