Ham's F-12K Dry Powder Media
- Catalog Number : CM1012、CM1013
- Number : CM1012
-
Size:
Qty : - Price : Request Inquiry
-
Introduction
Ham's F-12K (Kaighn's) Media is a modification of Ham's F-12 Media. It contains 2 mM L-glutamine and 1260 mg/L D-glucose. Ham's F-12K Media contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the Media to be supplemented with very low levels of serum or defined components, for primary human hepatocytes, rat and chicken liver cells.
Ham's F-12K Media contains no proteins or growth factors. Therefore, often supplemented with growth factors, Fetal Bovine Serum (FBS) or Specsure® Bovine Growth Serum. Ham's F-12K Dry Powder Media does not contain sodium bicarbonate, therefore adding 2.5 g sodium bicarbonate is needed for a 5% CO2 environment to maintain physiological pH.
Ham's F-12K Media contains no proteins or growth factors. Therefore, often supplemented with growth factors, Fetal Bovine Serum (FBS) or Specsure® Bovine Growth Serum. Ham's F-12K Dry Powder Media does not contain sodium bicarbonate, therefore adding 2.5 g sodium bicarbonate is needed for a 5% CO2 environment to maintain physiological pH.
General Information
| Cas No. | CM1012、CM1013 |
|---|---|
| Storage instruction | 2-8 ˚C |
- Sterility：Sterile-filtered
- Form：Powder
- Application (s)：Cell culture/ mammalian: suitable
- Impurities：Endotoxin, tested
- Components：
HEPES (-)
Sodium bicarbonate (-)
L-glutamine (2 mM)
D-glucose (1260 mg/ml)
Phenol red (3 mg/ml)
NOTES
- The net weight of this product is 11.15 g, and 1L Ham's F-12K medium can be prepared.
- Dissolve the Ham's F-12K Powder with ultrapure water for cell culture.
- Add 2.5 g sodium bicarbonate and dissolve fully.
- (Optional) Adjust pH with 1M NaOH solution or 1M HCl solution.
- Add ultrapure water for cell culture to the final volume of 1L.
- The prepared solution should be sterilized with 0.22 µm filters under aseptic condition.
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