AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody
- Catalog Number : A20251PI
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General Information
| Reactivity | Human, Mouse, Rabbit, Monkey, Procine |
|---|---|
| Application | WB, IF, IHC, ELISA |
| Host | Rabbit |
| Uniprot | Q13131/P54646 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Formula | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Dilution:
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Figure:
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Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
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Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1：500
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Western Blot analysis of KB cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1：500
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The picture was kindly provided by our customer
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The picture was kindly provided by our customer
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Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of rat-heart tissue.
1,AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).
3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK alpha (Phospho-Thr172) Antibody
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