Mycoplasma Removal Agent (100X)
- Catalog Number : CM1003
- Number : CM1003
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Size:
Qty : - Price : 5000 Inquiry
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Introduction
Mycoplasma contamination is a common issue in cell culture. It can alter the characteristics of cells. For instance, some mycoplasma species rapidly deplete arginine in the culture medium, competing with cells for nutrients, which can lead to slowed or halted cell growth. Additionally, mycoplasma can modify the enzyme profiles and membrane components of cells, causing chromosomal abnormalities or cytopathic changes. These effects can significantly compromise the reliability of experimental results obtained using contaminated cells.
Our product utilizes a combination of effective components that target mycoplasma ribosomes to inhibit protein synthesis and disrupt cell membrane integrity. It also acts on DNA helicase to inhibit the synthesis and replication of mycoplasma DNA. The ingredients can penetrate mammalian cell membranes, eliminating both extracellular and intracellular mycoplasma, ensuring that treated cells are not re-infected by intracellularly released mycoplasma. Within 1-2 weeks, over 90% of mycoplasma can be eradicated with minimal cytotoxic effects. Additionally, this product exhibits inhibitory effects on both Gram-negative and Gram-positive bacteria.
Our product utilizes a combination of effective components that target mycoplasma ribosomes to inhibit protein synthesis and disrupt cell membrane integrity. It also acts on DNA helicase to inhibit the synthesis and replication of mycoplasma DNA. The ingredients can penetrate mammalian cell membranes, eliminating both extracellular and intracellular mycoplasma, ensuring that treated cells are not re-infected by intracellularly released mycoplasma. Within 1-2 weeks, over 90% of mycoplasma can be eradicated with minimal cytotoxic effects. Additionally, this product exhibits inhibitory effects on both Gram-negative and Gram-positive bacteria.
General Information
| Application | Cell |
|---|---|
| Storage buffer | Store at -20°C. Avoid freeze-thaw. |
Product Features:
- High Efficacy: Exhibits broad-spectrum elimination against multiple mycoplasma strains.
- Rapid Action: Significantly reduces mycoplasma contamination in a short period.
- Safety: Non-toxic to cells, ensuring normal cell growth and function.
- Ease of Use: Simply add to the culture medium without the need for complex procedures.
Application:
- Suitable for various cell cultures, including animal cells, human cells, and stem cells.
- Ideal for use in research laboratories, pharmaceutical companies, and biotechnology firms during cell culture processes.
- Particularly beneficial for precision experiments and research requiring high-purity cell cultures.
Usage Instructions
- Thawing Instructions: After thawing, if any crystallization occurs, place the vial in a 37°C water bath for 10-15 minutes until completely dissolved. This will not affect the product's efficacy.
- Preparation of Working Medium: Add the product to the complete culture medium at a 1:100 ratio to prepare a 1X working medium.
- Cell Passage Instructions: During cell passaging, centrifuge to remove the culture supernatant, then resuspend the cells in the 1X working medium for normal culture and passaging for 1-2 weeks. Washing cells with PBS 1-2 times during passaging can enhance the efficiency of mycoplasma elimination.
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