Mycoplasma Detector
- Catalog Number : CM1001
- Number : CM1002
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Introduction
Mycoplasma contamination is a common issue in mammalian cell culture, potentially leading to inaccurate experimental results. Since 2013, high-impact journals like "Nature" have required mycoplasma testing for articles involving cell culture. This product utilizes a unique isothermal gene amplification technology, designed to provide a rapid, simple, and highly sensitive method for mycoplasma detection.
General Information
| Application | Cell |
|---|---|
| Assay principle | Observe the solution color in a bright environment. It is recommended to use a white paper as background. Purple solution color represents mycoplasma negative and sky blue color represents mycoplasma positive. |
| Storage instruction | Storage at -20 ℃ |
Product Advantages:
- Rapid Detection: The entire detection process takes only 1 hour, significantly reducing the weeks required by traditional culture methods.
- Easy Operation: No PCR inhibitors have been found in cell culture fluids to inhibit isothermal gene amplification, generally eliminating the need for sample pre-treatment.
- High Sensitivity: The sensitivity of isothermal gene amplification surpasses that of conventional PCR methods with 30 cycles.
- Intuitive Results: No electrophoresis needed; results can be directly observed by color change of the indicator.
- Low Equipment Requirements: The entire detection process requires only a water bath, eliminating the need for a PCR machine, electrophoresis tank, or gel imaging system.
Product Limitations:
- Identification Rate: The mycoplasma identification rate is relatively low, identifying approximately 99% of mycoplasma species, with about 1% risk of false negatives.
- Interference Factors: High concentrations of serum, blood products, divalent ions, and EDTA may interfere with the colorimetric results but can be removed by centrifugation and washing.
Detection Range:
This kit can detect at least the following 13 types of mycoplasma:
| Mycoplasma hyorhinis | Mycoplasma fermentans |
| Mycoplasma arginini | Mycoplasma hominis |
| Mycoplasma orale | Mycoplasma salivarium |
| Mycoplasma pirum | Acholeplasma laidlawii |
| Mycoplasma agalactiae | Mycoplasma bovis |
| Mycoplasma buccale | Mycoplasma arthritidis |
| Mycoplasma pulmonis |
The first 8 types are the most common mycoplasma contaminants in cell culture, accounting for approximately 98% of contaminated cells, while the above 13 types account for about 99% of contaminated cells.
Mycoplasma Detector provides a rapid, simple, and highly sensitive method for mycoplasma detection, suitable for laboratories needing quick detection of mycoplasma contamination in cell culture. Despite certain limitations, its high efficiency and simplicity make it an indispensable tool in cell culture laboratories.
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