General Information
| Reactivity | Human |
|---|---|
| Application | IF, IHC, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
P42574 |
|
Immunogen |
Recombinant human Caspase-3 protein (29-175AA) |
| Application |
IHC : 1:50-200 IF : 1:50-500 |
|---|
| Figure |
Figure 1 : Immunofluorescence staining of A549 cells at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Figure 2 : Immunohistochemistry of paraffin-embedded human colon cancer using A8925P at 1:100 dilution. |
|---|---|
| Purification Method | >95%, Protein G purified |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|---|
| Alias | Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12], CASP3, CPP32 |
|---|
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