Introduction
This kit allows for the determination of IFN-α concentrations in Human serum, plasma, tissue homogenates and other biological fluids.
General Information
| Reactivity | Human |
|---|---|
| Application | ELISA |
| Assay principle | The kit assay Human IFN-α level in the sample, use Purified Human IFN-α antibody to coat microtiter plate wells, make solid-phase antibody, then add IFN- α to the wells, Combined antibody which With HRP labeled, become antibody-antigen-enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IFN- α in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Sensitivity | The minimum detectable dose is typically less than 0.1 pg/mL. |
| Detection range | 1.25 pg/mL - 40 pg/mL |
| Sample type | Human serum, plasma, tissue homogenates and other biological fluids. |
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