Introduction
Rhodamine B is a useful fluorochrome for histology due to its fluorescent properties in ultraviolet light. It ejects an electron onto TiO2, which is then scavenged by O2 forming the O2- radical anion. This anion ultimately becomes the OH radical. A pH-independent process degrades Rhodamine B in TiO2 dispersions containing no dodecylbenzenesulfonate. The compound has been incorporated in a FRET system based on lanthanide-doped nanoparticles for monitoring biological effects. Rhodamine B has also been used as a mitochondrial probe to study the energetic state of multi-drug resistant and sensitive cells where the mitochondrial Rhodamine B concentration was precisely determined with the loss of fluorescence in the presence of MTT Formazan ，a fluorescence quencher. In addition, Rhodamine B has been observed to significantly reduce the number of human lip fibroblast cells in culture. Used for staining Negri bodies in tissue.
General Information
| Cas No. | 81-88-9 |
|---|---|
| Purity | ≥95% |
| Molecular Weight | 479.01 |
| Formula | C28H31ClN2O3 |
| Solubility | Rhodamine B is soluble in water (12 mg/ml), ethanol (70 mg/ml), acetone (slightly ), methyl cellosolve, and alcohol. |
| Storage instruction | Store at room temperature and protect from light |
| Alias | Brilliant Pink B;Rhodamine O;Tetraethylrhodamine;C.I. 45170 |
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