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Cell culture, COVID-19 
, COVID-19 Pseudovirus, Cell Line
ACE2-3xFLAG-HEK293T stable cell line
- Catalog Number : CL0021
- Number :
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Introduction
ACE2 is also called ACEH and is called angiotensin converting enzyme 2. The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidase, and is the functional receptor for SARS and HCoV-NL63 human coronavirus S glycoprotein. This ACE2-3xFLAG-HEK293T stable cell line expresses the ACE2-3xFLAG fusion protein, which can be used for ACE2 gene related research.
General Information
| Research topic | COVID-19 |
|---|
CONSTRUCTION
Stable Cell Line: The ACE2-3xFLAG-HEK293T stable cell line was obtained from lentivirus infected and multiple rounds of Puromycin screening. The lentivirus was packaged with pLV-ACE2-3FLAG plasmid, concentrated and purified, and then infected with HEK293T cells.
Vector Information
APPLICATIONS
ACE2-3xFLAG-HEK293T stable cell infected by Pseudovirus-SARS-CoV-2 (ACE #PV031), can be observed the expression of green fluorescent protein and detected the activity of luciferase.
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