Introduction
This ELISA kit uses Indirect-ELISA as the method to detect the HPIV-IgG in human serum (plasma). The micro ELISA plate provided in this kit has been pre-coated with purified HPIV antigen. Samples are added to the micro ELISA plate wells and the HPIV antibody in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugated Mouse anti human IgG antibody is added to each well and react with the compound to form “HPIV antigen- HPIV antibody-HRP antibody” compound. The TMB substrate is added to initiate the color developing reaction. The presence of HPIV-IgG can be determined according to the OD value after colorimetric assay with the Micro-plate Reader.
General Information
| Application | ELISA |
|---|---|
| Sample type | serum |
| Storage instruction | Store unopened at 2 to 8℃ for 12 months. Do not freeze |
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