Chemical, Inhibitor, Western Blotting, Protein Extraction
Protease Inhibitor Cocktail (without EDTA, Mini tablet)
- Catalog Number : A1061
- Number : A1061-s
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Size:
Qty : - Price : Request Inquiry
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Introduction
Generally, the production and degradation of endogenous proteins are maintained in balance. Therefore, the overall protein level in cells should also stay at a static state while the environmental factors are stable. However, the rapid loss in total protein amount due to the termination of protein synthesis would lead to the poor performance of protein extraction in vitro. The protease might also cause the degradation of all kinds of protein, including phosphorylated protein, when there is no protein inhibitor added in sample. Thus, using a combination of various small molecule inhibitors to inhibit the function of protease are recommended. Protease inhibitor cocktail can be used in mammalian tissue lysate and cell extract in order to increase the protein stability. ACE Biolab’s Protease Inhibitor Cocktail contains five inhibitors for different kinds of protease, which can protect the protein integrity effectively.
General Information
| Application | Extraction, Inhibitor |
|---|
COMPOSITION
|
AEBSF |
Serine protease |
Irreversible |
|
Aprotinin |
Serine protease |
Reversible |
|
Bestatin |
Aminopeptidases |
Reversible |
|
E-64 |
Cysteine proteases |
Irreversible |
|
Leupeptin |
Serine and cysteine proteases |
Reversible |
INSTRUCTION
- Can be used in Western Blot, Co-IP, pull-down assay, IF, IHC, kinase assay.
- Each Mini tablet can be directly added into 10 mL cell lysate.
- The 10X stock solution can be prepared by adding one tablet to 1 mL ddH2O. The stock solution can store at 4°C for more than 1 week and at -20°C for more than 4 weeks.
NOTE
- Vortex helps to increase the dissolution velocity of the tablet.
- Some of the DUB protease, e.g. ATAXIN-3, cannot be inhibited by common protease inhibitors such as E-64, AEBSF, Bestatin, Leupeptin and Aprotinin.
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