Introduction
This ELISA kit applies to the in vitro quantitative determination of Porcine cTnT/TNNT2
concentrations in serum, plasma and other biological fluids.
concentrations in serum, plasma and other biological fluids.
General Information
| Reactivity | Porcine |
|---|---|
| Assay principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine cTnT/TNNT2. You can calculate the concentration
of Porcine cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve. |
| Sensitivity | 9.38 pg/mL |
| Detection range | 15.63-1000pg/mL |
| Sample type | serum, plasma,cell lysates,tissue homogenates, cell culture supernatant and other biological fluids. |
| Storage instruction | -20˚C and 4˚C for 6 months |
CONTENTS and STORAGE
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