Introduction
Testosterone is a hormone found in humans, as well as in other animals. The testicles primarily make testosterone in men. Women’s ovaries also make testosterone, though in much smaller amounts. Testosterone production starts to increase significantly during puberty, and begins to dip after age 30 or so [1]. Testosterone is most often associated with sex drive, and plays a vital role in sperm production. But it also affects bone and muscle mass, the way men store fat in the body, and even red blood cell production. A man’s testosterone levels can also affect his mood. Testosterone is used as a medication for the treatment of males with too little or no natural testosterone production, certain forms of breast cancer, and gender dysphoria in transgender men. This is known as hormone replacement therapy (HRT) or testosterone replacement therapy (TRT), which maintains serum testosterone levels in the normal range. Decline of testosterone production with age has led to interest in androgen replacement therapy [2].
[1] Mooradian, A. D., Morley, J. E., & Korenman, S. G. (1987). Biological actions of androgens. Endocrine reviews, 8(1), 1-28.
[2] Myers, J. B., & Meacham, R. B. (2003). Androgen replacement therapy in the aging male. Reviews in urology, 5(4), 216.
his ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine and saliva. Please contact tech-support for other sample type detection.
[1] Mooradian, A. D., Morley, J. E., & Korenman, S. G. (1987). Biological actions of androgens. Endocrine reviews, 8(1), 1-28.
[2] Myers, J. B., & Meacham, R. B. (2003). Androgen replacement therapy in the aging male. Reviews in urology, 5(4), 216.
his ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine and saliva. Please contact tech-support for other sample type detection.
General Information
| Reactivity | Universal |
|---|---|
| Application | ELISA |
| Conjugate | HRP |
| Sensitivity | 0.17 ng/ml. |
| Detection range | 0.31-20 ng/ml |
| Sample type | Serum, plasma, saliva and urine |
| Cas No. | E0028 |
| Storage instruction | -20˚C and 4˚C for 6 months |
|
Concentration(ng/ml) |
20 |
10 |
5 |
2.5 |
1.25 |
0.62 |
0.31 |
0 |
|
OD |
0.269 |
0.545 |
0.740 |
1.006 |
1.299 |
1.608 |
1.919 |
2.426 |
REFERENCE VALUES
Samples from different species were evaluated for the presence of T in this assay.
|
|
Reference range of T in different species(ng/ml) |
||||||
|
Sample type |
Human |
Female Rat |
Female Mouse |
Chicken |
Porcine |
Sheep |
Cattle |
|
Serum(n=10) |
1.43-9.69 |
4.97-6.58 |
0.36-0.62 |
4.61-8.45 |
0.75-1.99 |
0.94-2.72 |
0.85-1.00 |
|
Plasma(EDTA)(n=10) |
0.69-8.05 |
1.01-1.08 |
ND |
1.51-2.53 |
0.30-0.97 |
0.77-3.73 |
0.83-1.44 |
|
Urine(n=5) |
3.88-21.05 |
- |
- |
- |
- |
- |
- |
|
Saliva(n=5) |
0.84-1.67 |
- |
- |
- |
- |
- |
- |
The above values were all from normal healthy samples.
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