Enzyme, RNA reagent/RT-PCR, qPCR
ACE Script II One Step qRT-PCR Kit (Probe)
- Catalog Number : EP2026
- Number : EP2026
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Size:
Qty : - Price : Request Inquiry
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Introduction
The ACE Script II One Step qRT-PCR Kit is specially designed for qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The HiScript II Reverse Trancriptase and hot-start Champagne Taq DNA Polymerase contained this kit enables high-sensitive total RNA detection (as little as 1 pg). The ACE Script II One Step qRT-PCR Kit is a master mix system. The 2× One Step Q Probe Mix contains an opimized buffer and dNTP/dUTP mix and is suitable for high-sensitive detection systems based on fluorescence labelled probes (i.e. TaqMan ).
General Information
| Application | qPCR |
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CONTENTS
|
Component |
EP2026 250 rxn (20 μl/rxn) |
|
RNase free ddH2O 2× One Step Q Probe Mix a One Step Q Probe Enzyme Mix b 50× ROX Reference Dye 1 c 50× ROX Reference Dye 2 c |
1.25 ml × 2 1.25 ml × 2 250 µl 100 µl 100 µl |
a. contains dNTP Mix and Mg2+ .
b. contains HiScript II Reverse Transcriptase, RNase inhibitor, and Champagne Taq DNA Polymerase.
c. Used to rectify the error of fluorescence signals between different wells. Use 50× ROX Reference Dye 1 for ABI 7900HT/ 7300 Real-Time PCR and System and StepOne PlusTM；Use 50× ROX Reference Dye 2 for ABI 7500, 7500 Fast Real-Time PCR System and Stratagene Mx3000P. Don't use ROX for neither Roche nor Bio-Rad Real-Time PCR instruments.
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