Western Blotting, Protein Quantification
Bradford protein assay (5X)
- Catalog Number : A1034
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Introduction
Bradford protein assay (5X) is a fast protein quantification method. It based on the method of Bradford, coomassie-binding with protein in an acidic solution. The measurement of absorbance shifts from 465 nm (brown color) to 595 nm (blue color) when binding to protein occurs. In addition, the coloration differs greatly depending on the basic and aromatic amino acid residues of protein. Bradford protein assay (5X) provides a wide protein quantification range from 1-1,000 μg/ml and the measured absorbance at 595 nm is stable for 5 to 60 minutes after the binding reaction starts.
General Information
| Storage instruction | Storage at 4˚C |
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