Human IL-6(Interleukin 6) ELISA Kit

Introduction

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

Application	ELISA
Conjugate	HRP
Assay principle	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibod
Sensitivity	4.69 pg/mL
Detection range	7.81—500 pg/mL
Sample type	serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction	-20˚C and 4˚C for 6 months
Alias	IL6, BSF2, HGF, HSF, IFNB2, CDF

INTRODUCTION

This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma and other biological fluids.

SPECIFICATION

Sensitivity: 4.69 pg/mL
Detection Range: 7.81-500 pg/mL
Specificity: This kit recognizes Human IL-6 in samples. No Significant cross-reactivity or interference between Human IL-6 and analogues was observed.
Repeatability: Coefficient of variation is < 10%.

PRINCIPLE of KIT

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with

an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with

the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish

Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed

away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection

antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the

addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a

wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the

concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.