Introduction
General Information
| Reactivity | Mouse |
|---|---|
| Application | ELISA |
PRINCIPLE of KIT
This kit employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with mouse IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for mouse IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of mouse IgG in samples. The color development is stopped and the intensity of the color is measured.
SPECIFICATION
Sensitivity: 29 ng/mL.
Detection Range: 125 – 8000 ng/mL
Specificity: This kit recognizes mouse IgG in samples. No significant cross-reactivity or interference between mouse IgG and analogues was observed.
Intra-assay Precision (Precision within an assay): CV% <8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% <10%
Three samples of known concentration were tested in twenty assays to assess.
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