Introduction
This ELISA kit is used to measure phosphorylated human, mouse, and rat ERK1 (T202 / Y204) and ERK2 (T185 / Y187) in cell lysates
General Information
This ELISA kit contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat ERK1 (T202 / Y204) and ERK2 (T185 / Y187) in cell lysates. An immobilized capture antibody specific for ERK1 / ERK2 binds both phosphorylated and unphosphorylated ERK1 / ERK2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.
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Assay Type |
Solid Phase Sandwich ELISA |
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Sample Volume Required |
Cell lysates (100 µL) |
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Sufficient Materials |
Kits available for two 96-well plates |
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