| Introduction |
Agarose is a polysaccharide and is most commonly used technique for gel electrophoresis in biochemistry and molecular biology to separate molecules such as DNA, RNA and protein.
The buffer chose to separate DNA in horizontal agarose gels is either Tris-acetate or Tris-borate buffer. RNA molecules are separated in denaturing agarose gels containing formaldehyde and performed in MOPS buffer. The efficient separation of DNA fragments of a wide size range is possible by adjusting the agarose concentration accordingly. |
.png)