Primary Antibody

iNOS/NOS2 Polyclonal Antibody

  • Catalog Number : A20241PI
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General Information

Reactivity Human, Mouse, Rabbit
Application WB, IF, IHC, ELISA
Host Rabbit
Uniprot P35228
Immunogen The antiserum was produced against synthesized peptide derived from human iNOS. AA range:117-166
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Formula Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Alias NOS2; NOS2A; Nitric oxide synthase; inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II;

Figure

Western Blot analysis of SH-SY5Y cells using NOS2 Polyclonal Antibody diluted at 1500

 

Western blot analysis of 3T3 lysis using NOS2 antibody. Antibody was diluted at 1:500

 

Immunofluorescence analysis of mouse-lung tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

 

Immunofluorescence analysis of mouse-lung tissue. 1,NOS2 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

 

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,NOS2 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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