General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, IHC, ELISA, IP |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
P40763 |
| Immunogen | Synthesized peptide derived from human Stat3 around the phosphorylation site of Y705. |
| Application |
WB : 1:500-2000 IHC : 1:100-300 ELISA : 1:20000 IP : 2-5ug/mg |
|---|
| Figure |
Figure 1 : Western blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA using A8855P.
Figure 2 : Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using A8855P (left) or the same antibody preincubated with blocking peptide(right).
Figure 3 : Immunofluorescence staining of methanol-fixed Hela cells using A8855P.
Figure 4 : Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using A8855P. |
|---|
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%20Antibody/A8855P%20Phospho-STAT3%20(S727)%20Antibody-IF-1.jpg)
| Purification Method | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy |
|---|---|
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|---|
| Alias | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 |
|---|
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