General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, IHC, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
O95169 |
| Immunogen | Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial protein (29-132AA) |
| Application |
WB : 1:500-5000 IHC : 1:200-500 |
|---|
| Figure |
Figure 1 : Western Blot analysis using NDUFB8 antibody at 2ug/ml Lane 1 : NIH/3T3 whole cell lysate Lane 2 : Rat brain tissue Lane 3 : Mouse heart tissue
Figure 2 : IHC image of A7768P diluted at 1:400 and staining in paraffin-embedded human heart tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Figure 3 : IHC image of A7768P diluted at 1:400 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system. |
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| Purification Method | >95%, Protein G purified |
|---|---|
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Alias | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (Complex I-ASHI) (CI-ASHI) (NADH-ubiquinone oxidoreductase ASHI subunit), NDUFB8 |
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