COVID-19 
, COVID-19 Pseudovirus, Pseudovirus, SARS-CoV-2
Pseudovirus-SARS-COV-2 (T20N)
- Catalog Number : PV053
- Number :
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Size:
Qty : - Price : Request Inquiry
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Introduction
ACE Biolabs used the lentiviral packaging system to package the SARS-CoV-2 (2019-nCoV, COVID-19 virus) T20N mutation protein as a surface capsid glycoprotein and containing GFP and Luciferase dual reporter genes as a pseudovirus model. The virus can infect cell lines overexpressing the ACE2 gene. Researchers can determine the infection efficiency by observing GFP through a microscope and detecting the value of Luciferase.
General Information
| Assay principle | Research use only.
Recommended amount: 2-10 μl/well of 96-well plate. According to experimental conditions, it can be adjusted. The infection efficiency of pseudoviruses highly depends on cell types. It is recommended to conduct a pre-experiment before the formal experiment to determine the optimal amount of virus. |
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| Storage instruction | The product can be stored at -80°C or below for 6 months. Avoid repeated freezing and thawing cycles. |
IMAGE:
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HEK293-ACE2-OE cell infected by Pseudovirus-SARS-CoV-2, can be observed the expression of green fluorescent protein and detected the activity of luciferase.
NOTE
- The pseudovirus-SARS-CoV-2 we provided is a replication-defective virus, that is, the virus does not use the host cell to generate new virus particles after infecting the target cells.
- The experimental operation needs to be carried out in the BSL-2 laboratory and Class II biological safety cabinet, and wear personal protective equipment such as laboratory clothes, masks and gloves.
- If this product is accidentally spilled during the experiment, please use disinfectant to inactivate it immediately. If it splashes on the eyes, skin or other body parts, immediately rinse with plenty of water.
- The experimental waste generated by using this product needs to be autoclaved and treated according to the medical waste treatment requirements.
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