Introduction
This ELISA kit applies to the in vitro quantitative determination of Human PDGF-AB concentrations in serum, plasma and other biological fluids.
General Information
| Reactivity | Human |
|---|---|
| Application | ELISA |
| Assay principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-AB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-AB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-AB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-AB. You can calculate the concentration of Human PDGF-AB in the samples by comparing the OD of the samples to the standard curve. |
| Sensitivity | 18.75 pg/mL |
| Detection range | 31.25-2000 pg/mL |
| Storage instruction | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received. |
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