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CRB1 Polyclonal Antibody
CRB1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CRB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CRB1 Polyclonal Antibody
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRB1 antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CRB1 at approximately 154KD. The expected band size for CRB1 is at 154KD.
CRB1 Polyclonal Antibody
CRB1 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CRB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CRB1 Polyclonal Antibody
CRB1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CRB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CRB1 Polyclonal Antibody
CRB1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- CRB1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CRB1 Polyclonal Antibody
Overlay histogram showing U87 cells stained with (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CRB1 Antibody (1μg/1x106 cells) for 30 min at 20°C. Goat Anti-Rabbit IgG, DyLight 488 (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CRB1 Polyclonal Antibody
CRB1 Polyclonal Antibody
CRB1 Polyclonal Antibody
CRB1 Polyclonal Antibody
CRB1 Polyclonal Antibody
CRB1 Polyclonal Antibody

Antibody, Primary Antibody

CRB1 Polyclonal Antibody

  • Catalog Number : A540107
  • Number : A540107
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General Information

Reactivity Human
Application WB, ELISA, FC, ICC, IHC-p, IHC-F
Host Rabbit
Clonality Polyclonal
Uniprot P82279
Immunogen E. coli-derived human CRB1 recombinant protein
Assay principle Western blot, 0.1-0.5μg/ml
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml,
Immunocytochemistry, 0.5-1μg/ml,
Flow Cytometry, 1-3μg/1x106 cells,
Direct ELISA, 0.1-0.5μg/ml
Storage instruction At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Alias crumbs homolog 1 (Drosophila)；LCA8

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