Chemical, Cell culture, Cell staining, Cell staining
7-Aminoactinomycin D (7-AAD)
- Catalog Number : C3317
- Number : C3317-1mg
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Introduction
A fluorescent DNA dye for apoptosis and flow cytomtery studies.
7-Aminoactinomycin D ，an analog of the antibiotic actinomycin D ，is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase
7-Aminoactinomycin D ，an analog of the antibiotic actinomycin D ，is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase
General Information
| Application | FCM, Cell, FC |
|---|---|
| Cas No. | 7240-37-1 |
| Purity | ≥97%(HPLC) |
| Molecular Weight | 1270.43 |
| Formula | C62H87N13O16 |
| Solubility | Soluble in DMSO (20 mg/ml), ethanol, chloroform (1 mg/ml), and dichloromethane (20 mg/ml). |
| Storage instruction | Store at -20℃ |
| Alias | 7-AAD; 7-AminoActinomycin D;7-AAD |
| Melting Point |
252-253° C |
|---|
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