Introduction
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been precoated with an antibody specific to Porcine PP. Standards or samples are added to the micro ELISA plate wells
and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PP and
Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.
Free components are washed away. The substrate solution is added to each well. Only those wells that contain
Porcine PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical
density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is
proportional to the concentration of Porcine PP. You can calculate the concentration of Porcine PP in the
samples by comparing the OD of the samples to the standard curve.
and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PP and
Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.
Free components are washed away. The substrate solution is added to each well. Only those wells that contain
Porcine PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzymesubstrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical
density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is
proportional to the concentration of Porcine PP. You can calculate the concentration of Porcine PP in the
samples by comparing the OD of the samples to the standard curve.
General Information
| Reactivity | Porcine |
|---|---|
| Application | ELISA |
| Sensitivity | 46.88 ng/ml. |
| Detection range | 78.13-5000 ng/ml. |
| Sample type | serum, plasma, tissue, cell lysates, cell culture supernatant or other biological fluids |
| Storage instruction | -20˚C and 4˚C for 6 months |
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