Antibody, Antibody Sampler Kit
Effector Caspases and Substrates Antibody Sampler Kit
- Catalog Number : AK0149
- Number :
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Qty : - Price : Request Inquiry
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Introduction
Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1), Caspase-6 (Mch2), and Caspase-7 (CMH-1, Mch3, ICE-LAP3) are effector caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 (ICE-LAP6, Mch6) are cleaved and activated. The activated upstream caspases further process downstream executioner caspases by cleaving them into activated large and small subunits, thereby initiating a caspase cascade leading to apoptosis. PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization. Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis. Type-B lamins consist of lamin B1 and B2, encoded by separate genes. Lamin B1 is also cleaved by caspases during apoptosis.
General Information
| Reactivity | Human, Mouse, Rabbit |
|---|---|
| Application | WB, IHC, ELISA |
| Host | Rabbit |
| Storage instruction | Store at -20℃. Avoid freeze / thaw cycles. |
| Research topic | Regulation of Apoptosis |
PRODUCT INCLUDES
|
Cat No. |
Product name |
Quantity |
Applications |
Reactivity |
Host |
|
A340458 |
Caspase-3 Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340460 |
Caspase-6 Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340462 |
Caspase-7 Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human |
Rabbit |
|
A340581 |
Lamin A/C Polyclonal Antibody |
20μL |
WB, IHC, IF, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340582 |
Lamin B1 Polyclonal Antibody |
20μL |
WB, IHC, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A340740 |
PARP-1 Polyclonal Antibody |
20μL |
WB, ELISA |
Human, Mouse, Rat |
Rabbit |
|
A1013s |
Goat Anti-Rabbit IgG (H+L) (peroxidase/HRP conjugated) |
120μL |
WB, ELISA |
Rabbit |
Goat |
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