YAP (phospho Ser127) Polyclonal Antibody
- Catalog Number : A20393PI
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General Information
| Reactivity | Human, Mouse, Rabbit, Monkey |
|---|---|
| Application | WB, IF, IHC, ELISA |
| Host | Rabbit |
| Uniprot | P46937 |
| Immunogen | synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142 |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Formula | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
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Immunofluorescence analysis of rat-heart tissue. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
-if-136.jpg)
Immunofluorescence analysis of rat-heart tissue. 1,YAP (phospho Ser127) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western Blot analysis of various cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Cytoplasmic and Nuclear Fractionation kit
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Western Blot analysis of MCF7 cells using Phospho-YAP (S127) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Cytoplasmic and Nuclear Fractionation kit
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