Western Blotting, Protein Extraction

100X Phosphatase Inhibitor Cocktail (A+B)

  • Catalog Number : A1030
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General Information

Application Extraction
Description 

Phosphatase Inhibitor Cocktail is used to protect phosphorylated proteins from dephosphorlation. We provide a set of phosphatase inhibitors and divided into 2 tube, aqueous and DMSO groups, respectively.

Formulation 
Tube Ingredient Target 100X Conc.
A (aqueous) Sodium Tartrate Acid phosphatases 400 mM
Sodium Fluoride Acid phosphatases 100 mM
Sodium Molybdate Acid and phosphoprotein phosphatases 115 mM
Sodium Orthovanadate Alkaline phosphatases, PTPs, ATPase 100 mM
Imidazole Alkaline phosphatases 200 mM
B (DMSO) Cantharidin Ser/Thr phosphatases 500 μM
(-)-p-Bromotetramisole oxalate Alkaline phsophatases 2.5 mM
Microcystin LR PP1 and PP2A 500 nM
Protocol

Before extraction, dilute Phosphatase Inhibitor A and B with 1X RIPA Lysis Buffer in 1: 100 ( v/v ) ratio. 

Related Product

1X RIPA Lysis Buffer

5X RIPA Lysis Buffer

100X Protease Inhibitor Cocktail (EDTA-free)

Storage -20 ℃ for 2 years
Alias N/A

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