General Information
| Reactivity | Human, Mouse |
|---|---|
| Application | WB, IF, IHC, ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
P01375 |
| Immunogen | Recombinant Human Tumor necrosis factor protein (77-233AA) |
| Application |
WB : 1:2000-5000 IHC : 1:20-200 IF : 1:50-200 |
|---|
| Figure |
Figure 1 : Western Blot analysis using TNF antibody at 4ug/ml Lane 2 : Mouse heart Lane 3 : Mouse liver
Figure 2 : Immunohistochemistry of paraffin-embedded human colon cancer using A3857P at dilution of 1:100
Figure 3 : Immunohistochemistry of paraffin-embedded human breast cancer using A3857P at dilution of 1:100
Figure 4 : Immunofluorescence staining of HepG2 cells with A3857P at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
|---|
| Purification Method | >95%, Protein G purified |
|---|---|
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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| Alias | Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF); Intracellular domain 1 (ICD1); Intracellular domain 2 (ICD2); C-domain 1; C-domain 2; Tumor necrosis factor, soluble form], TNF, TNFA TNFSF2 |
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