General Information
| Reactivity | Human |
|---|---|
| Application | WB, IHC, ELISA, IP |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Non-conjugation |
| Product Type | Primary Antibody |
|---|---|
|
Uniprot No. |
P40763 |
| Immunogen | A synthesized peptide derived from human Phospho-STAT3 (S727) |
| Application |
WB : 1:500-5000 IHC : 1:500-200 IP : 1:200-1000 IP : 2-5ug/mg |
|---|
| Figure |
Figure 1 : Western Blot anaylsis of A549 whole cell lysate(treated with EGF or not) using A8856P at 1.88ug/ml
Figure 2 : IHC image of A8856P diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 : Immunoprecipitating Phospho-STAT3 in 293 whole cell lysate treated with Calyculin A. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) |
|---|
%20Antibody/A8856P%20Phospho-STAT3%20(S727)%20Antibody-WB.jpg)
%20Antibody/A8856P%20Phospho-STAT3%20(S727)%20Antibody-IHC-1.jpg)
%20Antibody/A8856P%20Phospho-STAT3%20(S727)%20Antibody-IP.jpg)
| Purification Method | Affinity-chromatography |
|---|---|
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|---|
| Alias | Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3 |
|---|
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